AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |
Back to Blog
![]() A ready-to-use formatno mixing, heating, or reducing required. Nine polypeptides that resolve into sharp, tight blue bands in the range of 4200 kDa (depending upon the buffer system) An excellent indicator for judging Western transfer efficiency. Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2. The SeeBlue Pre-Stained Standard can be used with NuPAGE, tris-glycine and tricine gels. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits ![]() Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) 2-D SDS-PAGE protein standards provide calibrated references for protein pI and molecular weight in the second dimension. Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence IEF and 2D protein standards are a mixture of native proteins with isoelectric points (pI) ranging from 4.45 to 9.6, providing reproducible pI calibration in native PAGE or agarose IEF gels. It can be used with NuPAGE, tris-glycine, and tricine gels. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range The SeeBlue Plus2 Pre-Stained Standard contains 10 proteins: 8 blue-dyed and 2 with contrasting colors, providing easier band identification. If your transfer conditions are good, then the majority of your protein will be on the first membrane (closest to the gel). Then, go ahead and blot both membranes (be sure to label which was farthest away from the gel). Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) To test is your transfer is too long, try inserting two membranes into your transfer stack, one right behind the other. Visible monitoring of gel separation and transfer efficiency Use loading volumes listed below to obtain the best results: Gel. There is no need to heat or add reducing agent. Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins The SeeBlue Plus2 Pre-Stained Standard is supplied ready-to-use.
0 Comments
Read More
Leave a Reply. |